Malaria epidemiology in mersin province, Turkey from 2002 to 2011.

Background Malaria is an infectious disease caused by Plasmodium spp. with high morbidity and mortality in human in tropical and subtropical regions. In recent years, number of malaria cases has been significantly reduced because of fight with the disease in Turkey. This study intended to investigate the malaria epidemiology in Mersin Province from 2002 to 2011 using data from the provincial Public Health Directorate. Methods Over ten years, 303573 blood samples were taken from the people by active and passive surveillance methods and blood smears were prepared. Smears were stained with Giemsa and examined under the microscope. Results Totally, 73 people including 44 male and 29 female were positive in terms of Plasmodium spp. It was determined that P. vivax observed in 67 cases while P. falciparum in 6 cases. Cases were mainly observed in 15 to 44 years old range, showed an increase between June-September periods and a significant decrease after 2006. Out of the 73 malaria cases, 54 cases were from Mersin Province and 13 cases were imported from another province of Turkey. Six cases were transmitted from abroad. Conclusion These results provide information about malaria epidemiology in an endemic area in Turkey and contribute its prevention in Mersin Province.


Introduction
alaria is a public health problem caused by Plasmodium species and transmitted by mosquitoes of Anopheles genus commonly seen in tropical and subtropical zones globally. People can be infected by infected mosquito bites, by blood transfusion, tissue transplantation and transplacental carry (1). There are more than one hundred Plasmodium species infect various mammals. In human, malaria is caused by P. M falciparum, P. malariae, P. ovale, P. vivax and P. knowlesi (2,3). Among those infectious spp., P. falciparum is the most common species throughout the world followed by P. vivax. P. falciparum is responsible for the majority of deaths while the other species cause a generally milder form of malaria that is rarely fatal (4). Although the most common Plasmodium species is P. vixax, malaria caused by P. falciparum transmitted from abroad have also been observed in recent years in Turkey (5). In 1957, Turkey participated to malaria eradication program organized by the World Health Organization. Since that date malaria eradication studies have been successful and in 1970 the number of cases has decreased to 1263. Malaria cases have been increased again since 1971. In 1977 and 1994, 115512 and 84345 cases have been observed with large outbreaks respectively (5, 6). In Turkey some epidemiological studies are conducted about malaria (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24). The aim of this study was to evaluate malaria cases diagnosed between 2002 and 2011 in the province of Mersin in Turkey.

Materials and Methods
The materials of this study were thick drop and thin blood smears prepared from 303573 people who were suspected having malaria by Mersin Public Health Directorate between 2002 and 2011. Plasmodium species were investigated under a light microscope magnification X1000 after preparation of blood samples from febrile and afebrile periods. Results were evaluated in terms of years, genders, age groups, seasons, locations and identified species. Locations, where malaria cases were detected in the province of Mersin are presented in Fig. 1.

Results
Blood samples were taken from 303573 individuals and malaria parasite has been identified in 73 persons (0.02%) including 44 male and 29 female over ten-years. Malaria cases were determined in 0-1 months and 65 years old periods and mostly between 15 to 44 years old people.      Tarsus  Silifke  Gülnar  2002  12  3  1  -2003  13  3  --2004  15 1

Discussion
Against the increasing diagnostic and treatment methods and also eradication programs, malaria is still an important infection today globally. While malaria causes moderate infections in people with a strong immune response, it can be fatal for baby, children and pregnant women (25). In Turkey, malaria is still a problem because it creates a burden on the economy and causes loss of work and power (24,26 Malaria can be seen in both male and female. But it is observed in our study frequently in males (60.27%) and the results of similar studies (7-9) support this data. It is thought that malaria cases were seen more in males due to they are more active than females in working life and exist in the external environment during the evening when mosquitoes are active. In 91.78% of malaria cases the agent was P. vivax and in 8.22% P. falciparum was responsible for the diseases. This result is compatible with the data of P. vivax is the only endemic species in Turkey (18,21,24,27). Although P. vivax is the only endemic species in Turkey, P. falciparum is responsible from some cases determined in the country and it is thought that it sourced from abroad (9,10,21,22). Malaria cases are frequently observed in people between the ages of 15-44 yr and mostly seen in people over the age of 15 yr (15,16,21). This shows that the results of other studies conducted on this topic in Turkey are compatible of our study result. It is considered that people over the age of 15 yr travel more than the others for the purpose of business, education and tourism. This situation is a factor increasing the probability of exposure to malaria vector by being outdoors for a long time.
In this study, malaria cases were determined mostly in June (24.65%), September (23.28%), November (16.43) and August (13.69%), respectively. More cases were occurred in the summer months than the other months as a result of ideality for mosquito breeding and the development, having a lot of tourism activity and people being in the outdoor environments (26,28,29). Additionally Mersin has subtropical climatic characteristics. So malaria cases were determined in all seasons in Mersin Province of Turkey.

Conclusion
Malaria cases were viewed throughout the year in Mersin Province because of the climate, the nature, the socio-economic structure and population mobility. It is recommended that malaria screening and vector control must be done throughout the year in Mersin Province and all of Turkey to maintain the decreasing the incidence of malaria.